水牛MFSD2A基因多态性及其与产奶性状的关联分析

【目的】研究水牛主要促进因子超家族成员2a(major facilitator superfamily domain containing 2a,MFSD2A)基因多态性,并筛选与水牛产奶性状相关联的SNP。【方法】采集383头健康水牛血液DNA,利用PCR扩增和Sequenom MassARRAY飞行时间质谱技术对SNP位点进行筛选及基因型检测,并进行遗传多样性、连锁不平衡(LD)和单倍型分析,以及对不同基因型和单倍型与水牛产奶性状进行关联分析。【结果】在水牛MFSD2A基因中检出7个SNPs位点,多态信息含量(PIC)均在0.25~0.40之间,属于中度多态,除g.8290 T＞C位点外,其余位点均处于Hardy-Weinberg平衡状态(P＞0.05);关联分析结果表明,这7个SNPs位点与305 d产奶量均显著关联(P＜0.05),且突变杂合子基因型的305 d产奶量是3种基因型中最低的,g.568 C＞G和g.701 A＞G位点与乳脂率显著关联(P＜0.05),乳脂率从大到小依次为突变纯合子＞野生纯合子＞突变杂合子,g.568 C＞G、g.3326 G＞A、g.8290 T＞C和g.8523 C＞G位点与乳蛋白率显著关联(P＜0.05),7个SNPs位点与乳尿素氮关联均不显著(P＞0.05);连锁不平衡和单倍型分析结果明,g.568 C＞G、g.701 A＞G和g.3203 T＞G位点可组成一个单倍型模块(Block 1),g.8523 C＞G和g.9138 G＞T位点可组成另一个单倍型模块(Block 2)。其中g.568 C＞G和g.701 A＞G、g.568 C＞G和g.3326 G＞A、g.8523 C＞G和g.9138 G＞T位点的r²分别为0.87、0.37和0.48,处于强连锁不平衡状态。Block 1中的H1(CAG)单倍型乳蛋白率显著高于H2(CAT)和H3(GCT)(P＜0.05),Block 2中的D3(GG)单倍型305 d产奶量极显著高于D1(CG)和D2(CT)(P＜0.01)。【结论】筛选出的MFSD2A基因7个SNPs位点与305 d产奶量均显著关联,g.568 C＞G和g.701 A＞G位点与乳脂率显著关联,g.568 C＞G、g.701 A＞G、g.3326 G＞A、g.8290 T＞C和g.8523 C＞G位点与乳蛋白率显著关联,这7个SNPs位点与乳尿素氮均无显著关联。纯合子的基因型在高305 d产奶量和乳脂率的选择上更有优势,H1(CAG)和D3(GG)单倍型是提高水牛305 d产奶量和乳蛋白率的有利单倍型。     

Introduction: That most remarkable of outside men – Harold Brookfield's intellectual legacy

Abstract: Harold Brookfield's academic career spans more than half a century, traversing small tropical and subtropical islands and mountainous uplands, focusing on people–environment relations and linking to a diversity of institutions and disciplines. His unwavering commitment to fieldwork at the local level and to comparative study is paralleled by a healthy scepticism with respect to academic trends and orthodoxy of any kind, whether intellectual or physical. It is the farmers of the developing world who are the source of much of his inspiration. His theoretical contributions are based essentially on his observations of their practices and his learning from their experiences. His academic insights into the processes of change in rural areas of Melanesia, East and South‐East Asia, Africa and South America, where small‐scale ecological studies are linked to global forces, are of lasting significance.     

The association of sexuality with plant traits and tuber yield in white yam

Summary One 3-to 5-year clonal and two recent seedling-derived populations of white yam cultivars were grown and studied for the association of sex, flowering intensity, number of fruits and seed yield with vegetative characters and tuber yield. Yield was lowest in non-flowering plants and increased in the following order: male, monoecious and female plants; with females yielding almost twice as much as the non-flowering plants. The plants produced from small tuber setts, with higher leaf virus infection, low plant leafiness either had low flowering intensity, fewer fruits and seeds or even failed to flower. The sequence and timing of vegetative and reproductive processes in relation to the difference in yield between male and female plants is discussed. There is a strong indication that the intensity of flowering and fruiting is lower in plants from previous vegetative propagation compared to recent seedling-serived clones.     

草类物料压缩试验研究中的突破性进展

在国内外草物料压缩试验研究的基础上,对新鲜草物料压缩流变进行试验研究,总结出了草物料压缩试验研究过程中几个带有突破性的进展,较系统地展示了草物料压缩研究逐步深入的历程和规律,对草物料压缩基础研究的发展有重要意义。在国外研究“闭式压缩”很长时间后,我国开始了“开式压缩”试验研究。从实验室的局部过程的试验研究,发展到压缩工程全过程的试验研究;从一般工程力学的研究,推进入到压缩过程流变学试验研究;从理论上的阶跃历程的流变学试验研究,跨越到工程上任意历程的流变学试验研究,将草物料压缩流变研究推向新阶段。最后又提出了变断面积的压缩流变试验研究,继续推进草物料压缩工程和草物料加工流变学发展。     

白羽番鸭ApoVLDL-Ⅱ基因内含子1的克隆及其多态与生长、肉质和脂肪性状的关联分析

以10周龄白羽番鸭为材料,对ApoVLDL-II基因内含子1进行克隆和SSCP检测,并分析其多态与生长、肉质和脂肪性状的关系。结果表明:白羽番鸭ApoVLDL-II基因内含子1序列全长1 362 bp,与家鸭和鸡的同源性分别为93.6%和69.2%;经SSCP分析,仅A4位点检测到多态,共3种基因型,序列比对发现在770 bp碱基之后插入/缺失1个AAAATCTTGTTTA序列和803 bp处发生A→G突变,该位点属中度多态,并偏离了Hardy-Weinberg平衡(P<0.05);关联分析显示,CC基因型个体的pH和胸肌水分显著低于DD基因型的(P<0.05),肌内脂肪(IMF)、血清甘油三酯(TG)和血清胆碱酯酶(ChE)则显著高于DD型的(P<0.05),CD基因型个体的胸肌水分显著低于DD基因型的(P<0.05),其他性状各基因型间的差异均不显著(P>0.05)。由此推测,白羽番鸭ApoVLDL-II基因内含子1的多态可能对肉质和脂肪代谢有着重要影响,CC基因型可能是脂肪沉积的有利基因型。     

生态公益林经营管理研究进展

生态公益林经营管理是一个全新的课题,生态效益补偿问题、效益评价、经营方案的编制等都是实现生态公益林合理经营的重要保障。通过对生态公益林经营管理现状的分析,提出生态公益林经营方案的编制、监测体系的建设、合理的开发利用以及区域生态公益林稳定性等是今后生态公益林经营管理重要研究方向。     

The pharmacokinetical study of plant alkaloid tetrandrine with a simple HPLC method in rabbits

A simple HPLC method was developed to quantify rabbit plasma tetrandrine (Tet) with propranolol (Pro) as internal standard. Based on the established method Tet and Pro were eluted at 7.1 and 12.0 min, respectively. It was shown that the concentration-time data of Tet fit the classical two-compartment model, no matter the drug was administered intravenously or orally to rabbits. The values of AUC_0 → ∞, clearance (Cl_0 → ∞), volume of distribution (Vd), and elimination half-life (t_1/2β) of Tet were 59861.149 ± 26962.196 μg/L ? min, 0.503 ± 0.173 L/min/kg, 179 ± 76.185 L/kg, and 283.808 ± 162.937 min for intravenous injection of 5 mg/kg, or 18986.217 ± 7462.308 μg/L ? min, 0.805 ± 0.267 L/min/kg, 110.284 ± 94.176 L/kg, and 732.919 ± 847.32 min for gavage administration of 10 mg/kg , respectively. The results indicate that Tet displays a limited absorption in intestinal tract, even though it has a favorable pharmacokinetic profile after oral or intravenous administration.     

舞毒蛾性信息素诱捕器应用技术研究

通过对舞毒蛾性信息素诱捕器应用技术研究表明:三角型粘胶板诱捕器、奶盒型诱捕器和圆筒型诱捕器对舞毒蛾的诱捕效果不同,三角型粘胶板诱捕器的诱虫效果最好,圆筒型诱捕器次之;诱捕器设置高度对诱捕效果有明显的影响,设置高度为5.5 m的诱捕器诱捕作用最强,3.5 m次之,1.5 m最差;雄蛾对人工合成信息素的昼夜反应节律发生在白天,从8∶00持续到22∶00,反应高峰在14∶00,反应模式为"单峰式-白天型"。     

云南野生动物肇事危害情况及对策探讨

云南被誉为"动物王国",自然条件优越,适于各种野生动物生息繁衍。随着保护力度的加大,其野生动物的数量猛增,与人的冲突日显突出,肇事危害频繁,损失严重。针对云南野生动物肇事危害的原因和特点,提出多渠道、综合治理的对策建议。     

芳香疗法在园林中的应用研究

介绍了芳香疗法的定义、发展过程以及国内外研究应用现状。芳香疗法具有疗效可靠、安全舒适、无毒副作用的特点。在防病疗疾、优化环境以及精神方面均有独特的功效。提出了“芳香植物专类园”、“夜花园”、“盲人植物园”、“保健绿地”等园林绿地的应用技术。